ARACHIDONIC METABOLISM PATHWAY Introduction Arachidonic Acid pathway describes the synthesis of different Prostanoids (PGs, TXs, Prostacyclin) and Leukotrienes, Cysteinyl Leukotrienes, and Lipoxanes. Steps of AA pathway Step – 01 (Activation of Phospholipase A 2 and release of Arachidonic Acid) Release of AA from phospholipid membrane is carried out by Phospholipase A2 Stimuli initiates the Ca+2 dependent translocation of cytosolic PLA2 to the membrane where it hydrolyses the AA and releases It into the cytosol. Step – 02 (Cyclooxygenase Pathway) Arachidonic acid is converted to Prostaglandin G2 and Prostaglandin H2 catalyzed by Cyclooxygenase (COX-1 & COX-2) enzyme. Prostaglandin H2 is then Converted to various Prostanoids by several enzymes- Thromboxane A2 ( Converted by Thromboxane A synthase ) Prostaglandin E2 ( Microsomal Prostaglandin E synthase and cytosolic prostaglandin E Synthase ) Prostaglandin F 2 alpha ( Prostaglandin F synthase ...
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